|Title||Integrative pipeline for profiling DNA copy number and inferring tumor phylogeny.|
|Publication Type||Journal Article|
|Year of Publication||2018|
|Authors||Urrutia, Eugene, Hao Chen, Zilu Zhou, Nancy R. Zhang, and Yuchao Jiang|
|Date Published||2018 06 15|
|Keywords||Algorithms, DNA Copy Number Variations, Genomics, High-Throughput Nucleotide Sequencing, Humans, Models, Genetic, Models, Statistical, Neoplasms, Polymorphism, Single Nucleotide, Sequence Analysis, DNA, Software|
Summary: Copy number variation is an important and abundant source of variation in the human genome, which has been associated with a number of diseases, especially cancer. Massively parallel next-generation sequencing allows copy number profiling with fine resolution. Such efforts, however, have met with mixed successes, with setbacks arising partly from the lack of reliable analytical methods to meet the diverse and unique challenges arising from the myriad experimental designs and study goals in genetic studies. In cancer genomics, detection of somatic copy number changes and profiling of allele-specific copy number (ASCN) are complicated by experimental biases and artifacts as well as normal cell contamination and cancer subclone admixture. Furthermore, careful statistical modeling is warranted to reconstruct tumor phylogeny by both somatic ASCN changes and single nucleotide variants. Here we describe a flexible computational pipeline, MARATHON, which integrates multiple related statistical software for copy number profiling and downstream analyses in disease genetic studies.Availability and implementation: MARATHON is publicly available at https://github.com/yuchaojiang/MARATHON.Supplementary information: Supplementary data are available at Bioinformatics online.
|Original Publication||Integrative pipeline for profiling DNA copy number and inferring tumor phylogeny.|
|PubMed Central ID||PMC6248831|
|Grant List||P01 CA142538 / CA / NCI NIH HHS / United States |
R01 HG006137 / HG / NHGRI NIH HHS / United States
T32 ES007018 / ES / NIEHS NIH HHS / United States
Integrative pipeline for profiling DNA copy number and inferring tumor phylogeny.