Single-nucleotide resolution analysis of nucleotide excision repair of ribosomal DNA in humans and mice.

TitleSingle-nucleotide resolution analysis of nucleotide excision repair of ribosomal DNA in humans and mice.
Publication TypeJournal Article
Year of Publication2019
AuthorsYang, Yanyan, Jinchuan Hu, Christopher P. Selby, Wentao Li, Askar Yimit, Yuchao Jiang, and Aziz Sancar
JournalJ Biol Chem
Volume294
Issue1
Pagination210-217
Date Published2019 01 04
ISSN1083-351X
KeywordsAnimals, DNA Repair, DNA, Ribosomal, Humans, Mice, RNA Polymerase I, RNA Polymerase II, Sequence Analysis, DNA, Transcription, Genetic
Abstract

The unique nucleolar environment, the repetitive nature of ribosomal DNA (rDNA), and especially the possible involvement of RNA polymerase I (RNAPI) in transcription-coupled repair (TCR) have made the study of repair of rDNA both interesting and challenging. TCR, the transcription-dependent, preferential excision repair of the template strand compared with the nontranscribed (coding) strand has been clearly demonstrated in genes transcribed by RNAPII. Whether TCR occurs in rDNA is unresolved. In the present work, we have applied analytical methods to map repair events in rDNA using data generated by the newly developed XR-seq procedure, which measures excision repair genome-wide with single-nucleotide resolution. We find that in human and mouse cell lines, rDNA is not subject to TCR of damage caused by UV or by cisplatin.

DOI10.1074/jbc.RA118.006121
Alternate JournalJ Biol Chem
Original PublicationSingle-nucleotide resolution analysis of nucleotide excision repair of ribosomal DNA in humans and mice.
PubMed ID30413533
PubMed Central IDPMC6322885
Grant ListP01 CA142538 / CA / NCI NIH HHS / United States
P30 ES010126 / ES / NIEHS NIH HHS / United States
R01 ES027255 / ES / NIEHS NIH HHS / United States
R35 GM118102 / GM / NIGMS NIH HHS / United States
Project: