|Title||Single-nucleotide resolution analysis of nucleotide excision repair of ribosomal DNA in humans and mice.|
|Publication Type||Journal Article|
|Year of Publication||2019|
|Authors||Yang, Yanyan, Jinchuan Hu, Christopher P. Selby, Wentao Li, Askar Yimit, Yuchao Jiang, and Aziz Sancar|
|Journal||J Biol Chem|
|Date Published||2019 Jan 04|
|Keywords||Animals, DNA Repair, DNA, Ribosomal, Humans, Mice, RNA Polymerase I, RNA Polymerase II, Sequence Analysis, DNA, Transcription, Genetic|
The unique nucleolar environment, the repetitive nature of ribosomal DNA (rDNA), and especially the possible involvement of RNA polymerase I (RNAPI) in transcription-coupled repair (TCR) have made the study of repair of rDNA both interesting and challenging. TCR, the transcription-dependent, preferential excision repair of the template strand compared with the nontranscribed (coding) strand has been clearly demonstrated in genes transcribed by RNAPII. Whether TCR occurs in rDNA is unresolved. In the present work, we have applied analytical methods to map repair events in rDNA using data generated by the newly developed XR-seq procedure, which measures excision repair genome-wide with single-nucleotide resolution. We find that in human and mouse cell lines, rDNA is not subject to TCR of damage caused by UV or by cisplatin.
|Alternate Journal||J Biol Chem|
|Original Publication||Single-nucleotide resolution analysis of nucleotide excision repair of ribosomal DNA in humans and mice.|
|PubMed Central ID||PMC6322885|
|Grant List||P01 CA142538 / CA / NCI NIH HHS / United States |
P30 ES010126 / ES / NIEHS NIH HHS / United States
R01 ES027255 / ES / NIEHS NIH HHS / United States
R35 GM118102 / GM / NIGMS NIH HHS / United States
Single-nucleotide resolution analysis of nucleotide excision repair of ribosomal DNA in humans and mice.